Journal: Science Advances

Article Title: Biofunctional lipid nanoparticles for precision treatment and prophylaxis of bacterial infections

doi: 10.1126/sciadv.adk9754

Figure Lengend Snippet: ( A ) Expression levels of CD80 + and CD86 + in BMDCs (CD11c + ) and ( B ) production of IL-6 and TNF-α of the cell supernatant measured by ELISA after different treatments (concentration, 2 μg ml −1 NPs) for 24 hours ( n = 3). ( C ) Schematics of experimental design to evaluate the in vivo short-term immune responses activated by a single dose of various LNPs (20 μg/20 g) via subcutaneous injection. ( D ) Percentage of CD80 + and ( E ) CD86 + cells (gated on CD11c + cells) in the LNs at day 3 after immunization ( n = 6). ( F ) Percentage of CD3 + T cells, ( G ) CD8 + T cells (gated on CD3 + T cells), and ( H ) CD4 + T cells (gated on CD3 + T cells) in the spleen at day 3 after immunization ( n = 6). Naive mice without immunization were used as control. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD11c, phycoerythrin (PE)–conjugated anti-mouse CD80, PE-conjugated anti-mouse CD86, allophycocyanin (APC)–conjugated anti-mouse CD3, PE-conjugated anti-mouse CD8a, and FITC-conjugated anti-mouse CD4 were purchased from Tonbo Biosciences, USA.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, In Vivo, Injection, Control

Journal: Acta Pharmaceutica Sinica. B

Article Title: Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

doi: 10.1016/j.apsb.2023.11.032

Figure Lengend Snippet: The immune activation effect of OAP2 in vivo . (A–B) Analysis of mature DC cells in mouse spleen. Expression of CD80 and CD86 quantitatively detected by flow cytometry (A), and statistical analysis (B). (C–D) Analysis of mature DC cells in mouse tumor site. Expression of CD80 and CD86 quantitatively detected by flow cytometry (C), and statistical analysis (D). (E–F) Analysis of cytotoxic T cells in mouse tumor. Expression of CD4 and CD8 quantitatively detected by flow cytometry (E), and statistical analysis (F). (G–N) Tumor tissue cytokine expression content measured by ELISA ( n = 3).

Article Snippet: The following antibodies and kits were used in the flow cytometry and ELISA analyses: CoraLite® Plus 488 Anti-Mouse CD4, CoraLite®568 Anti-Mouse CD3, APC Anti-Mouse CD86, FITC Plus Anti-Mouse CD11c, and PE Anti-Mouse CD80 (B7-1) were purchased from Proteintech.

Techniques: Activation Assay, In Vivo, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay